Campo DC | Valor | Idioma |
dc.contributor.author | Costa, Diego Luis | - |
dc.contributor.author | Carregaro, Vanessa | - |
dc.contributor.author | Lima Júnior, Djalma S. | - |
dc.contributor.author | Silva, Neide M. | - |
dc.contributor.author | Milanezi, Cristiane M. | - |
dc.contributor.author | Cardoso, Cristina Ribeiro | - |
dc.contributor.author | Giudice, Ângela | - |
dc.contributor.author | Jesus, Amélia Maria Ribeiro de | - |
dc.contributor.author | Carvalho Filho, Edgar Marcelino de | - |
dc.contributor.author | Almeida, Roque Pacheco de | - |
dc.contributor.author | Silva, João S. | - |
dc.creator | Costa, Diego Luis | - |
dc.creator | Carregaro, Vanessa | - |
dc.creator | Lima Júnior, Djalma S. | - |
dc.creator | Silva, Neide M. | - |
dc.creator | Milanezi, Cristiane M. | - |
dc.creator | Cardoso, Cristina Ribeiro | - |
dc.creator | Giudice, Ângela | - |
dc.creator | Jesus, Amélia Maria Ribeiro de | - |
dc.creator | Carvalho Filho, Edgar Marcelino de | - |
dc.creator | Almeida, Roque Pacheco de | - |
dc.creator | Silva, João S. | - |
dc.date.accessioned | 2012-03-05T18:37:25Z | - |
dc.date.available | 2012-03-05T18:37:25Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1935-2735 | - |
dc.identifier.uri | http://www.repositorio.ufba.br/ri/handle/ri/5510 | - |
dc.description | 11 p. | pt_BR |
dc.description.abstract | Background: Leishmania braziliensis is the main causative agent of cutaneous leishmaniasis in Brazil. Protection against
infection is related to development of Th1 responses, but the mechanisms that mediate susceptibility are still poorly
understood. Murine models have been the most important tools in understanding the immunopathogenesis of L. major infection and have shown that Th2 responses favor parasite survival. In contrast, L. braziliensis–infected mice develop strong Th1 responses and easily resolve the infection, thus making the study of factors affecting susceptibility to this parasite
difficult. Methodology/Principal Findings: Here, we describe an experimental model for the evaluation of the mechanisms
mediating susceptibility to L. braziliensis infection. BALB/c mice were inoculated with stationary phase promastigotes of L.
braziliensis, isolates LTCP393(R) and LTCP15171(S), which are resistant and susceptible to antimony and nitric oxide (NO), respectively. Mice inoculated with LTCP393(R) presented larger lesions that healed more slowly and contained higher
parasite loads than lesions caused by LTCP15171(S). Inflammatory infiltrates in the lesions and production of IFN-c, TNF-a,
IL-10 and TGF-b were similar in mice inoculated with either isolate, indicating that these factors did not contribute to the
different disease manifestations observed. In contrast, IL-4 production was strongly increased in LTCP393(R)-inoculated
animals and also arginase I (Arg I) expression. Moreover, anti-IL-4 monoclonal antibody (mAb) treatment resulted in
decreased lesion thickness and parasite burden in animals inoculated with LTCP393(R), but not in those inoculated with
LTCP15171(S). Conclusion/Significance: We conclude that the ability of L. braziliensis isolates to induce Th2 responses affects the
susceptibility to infection with these isolates and contributes to the increased virulence and severity of disease associated
with them. Since these data reflect what happens in human infection, this model could be useful to study the pathogenesis of the L. braziliensis infection, as well as to design new strategies of therapeutic intervention. | pt_BR |
dc.language.iso | en | pt_BR |
dc.title | BALB/c Mice Infected with Antimony Treatment Refractory Isolate of Leishmania braziliensis Present Severe Lesions due to IL-4 Production | pt_BR |
dc.title.alternative | PLoS Neglected Tropical Diseases | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
dc.identifier.number | v. 5, n. 3. | pt_BR |
Aparece nas coleções: | Artigo Publicado em Periódico (Faculdade de Medicina)
|