https://repositorio.ufba.br/handle/ri/9457 2026-05-05T01:23:25Z https://repositorio.ufba.br/handle/ri/44397 Título: Interação entre exercício físico e o tratamento com resveratrol nas alterações hepáticas induzidas pelo diabetes mellitus em um modelo de pós-menopausa experimental Autor(es): Amado, Larissa Cruz Primeiro Orientador: Soares, Telma de Jesus Abstract: Type 1 diabetes mellitus (T1DM) is characterized by hypoinsulinemia resulting from autoimmune destruction of pancreatic beta cells. Hyperglycemia can induce alterations in liver function and structure associated with increased oxidative stress and inflammation. However, liver alterations in T1DM have been less documented, especially in postmenopausal women with T1DM. The search for new strategies is necessary to improve or delay the progression of liver lesions induced by T1DM in the postmenopausal period. In this context, we evaluated the isolated and combined effects of physical exercise and resveratrol (RES) on liver enzyme levels, morphology, redox balance, and inflammation in hepatocytes of ovariectomized (OVX) rats with T1DM. Twenty-four rats were OVX, and diabetes was induced by injection of streptozotocin (STZ/40 mg/kg). Next, the rats were divided into 4 groups (n=6/each): OSD, sedentary diabetic OVX rats; OTD, trained diabetic OVX rats; OSD+RES, sedentary diabetic OVX rats + RES; OTD+RES, trained diabetic OVX rats + RES. The OTD and OTD+RES groups underwent 8 weeks of exercise. Treatment with RES was performed by intraperitoneal injection, at a dose of 5 mg/kg/day, 5 days/week, for 8 weeks in the OSD+RES and OTD+RES groups. The rats were euthanized and the liver was removed for histological, morphometric, immunohistochemical, and oxidative stress studies. The results demonstrate that the interaction of RES with physical training preserved the structure of the liver tissue and reduced total nitrite levels, hepatic expression of NF-κB, nitrotyrosine, and TGF-β. However, in isolation, RES and physical training increased glycogen content, GPx enzyme activity, and reduced laminin immunostaining in the liver tissue of rats in the SDO+RES and OTD groups. RES alone decreased TBARS levels and increased SOD enzyme activity in the liver of OSD+RES rats. In conclusion, our results show that exercise and RES, separately, have hepatoprotective effects, but the interaction of these therapeutic strategies induced additional beneficial effects in the liver of OVX rats with T1DM. Editora / Evento / Instituição: Universidade Federal da Bahia Tipo: Dissertação 2025-12-16T00:00:00Z https://repositorio.ufba.br/handle/ri/43915 Título: Análise in silico de fatores de virulência de Chlamydia psittaci com potencial aplicação no desenvolvimento de vacinas e estudo do MHC de psitacídeos endêmicos do Brasil Autor(es): Teixeira, Sabrina Barbosa Primeiro Orientador: Bastos, Bruno Lopes Abstract: Chlamydia psittaci is an obligate intracellular pathogen of major zoonotic importance, widely associated with avians of the group Psittaciformes, with the potential to cause avian chlamydiosis and psittacosis in humans. The close relationship between these hosts and the infectious agent represents both a sanitary and conservation concern. The study of the Major Histocompatibility Complex (MHC) in psittacine species can serve as an important model for investigating host-pathogen interactions and the adaptive immune response of these avians, contributing to the development of strategies for the conservation of the group. This study aimed to investigate in silico the MHC of psittacine species endemic to Brazil and to perform the prediction of virulence factors and potential vaccine targets against C. psittaci. In the first chapter, 39 MHC class II sequences from Forpus passerinus and Amazona aestiva were identified and analyzed, with phylogenetic analyses indicating functional relationships with mammalian DRB and avian BL loci. Epitope prediction of the MOMP protein revealed regions with high binding affinity to MHC II molecules, including one shared between avians and mammals, suggesting potential for the development of broad-spectrum immunogens. In the second chapter, the complete proteome of a C. psittaci strain was analyzed for the identification of proteins associated with virulence, subcellular localization, and protein-protein interactions (PPI), highlighting molecular targets relevant to understanding pathogenesis and vaccine design. The results reinforce the applicability of in silico approaches in the immunogenetics of non-model species and in the search for new strategies for the prevention and control of avian chlamydiosis. Editora / Evento / Instituição: Universidade Federal da Bahia Tipo: Dissertação 2025-11-17T00:00:00Z https://repositorio.ufba.br/handle/ri/43221 Título: Análise da metilação do DNA em amostras gástricas e associação com infecção por Helicobacter pylori Autor(es): Sandes, Lidiane Ferraz Primeiro Orientador: Marques, Cintia Rodrigues Abstract: Helicobacter pylori infection is a significant risk factor for the development of inflammatory and neoplasic changes in the stomach, contributing to the pathogenesis of gastric diseases, particularly through epigenetic mechanisms. This experimental study aimed to examine the DNA methylation profile of the IL6, SOCS1, MGMT, and MLH1 genes in gastric mucosal samples, considering the presence of H. pylori infection and the detection of the virulence gene cagA. Samples underwent DNA extraction, followed by sodium bisulfite conversion and analysis using the MS-HRM technique. IL6 methylation was categorized into 0-25% and 25-50%, with stratification by sex, age, and bacterial strain status. Statistical analyses included the Student’s t-test to compare mean methylation levels between H. pylori-positive and -negative groups, and chi-square or Fisher’s exact test, as appropriate, considering p < 0,05 as the significance threshold for associations between categorical variables. The results indicated that infected individual were twice as likely to exhibit moderate methylation of the IL6 gene compared to non-infected individuals (OR = 2.00; 95% CI: 0.56–7.62; p = 0.284), and those infected with cagA-positive strains had a higher risk compared to those with cagA- negative strains (OR = 1.56; 95% CI: 0.41–5.85; p = 0.508), although these differences were not statistically significant. Among men, a significant association was observed (p = 0.035), with 100% of infected individuals showing moderate methylation versus 40% in the non- infected group; no significant difference was found among women (p = 0.765). Methylation of the SOCS1 and MLH1 genes was not detected in any of the samples, regardless of infection status, while MGMT methylation was observed in only three individuals (6.82%), all at low levels, preventing statistical analysis. Although no statistically significant association was observed with cagA status, all men infected with cagA-positive strains exhibited moderate methylation. These findings suggest that H. pylori infection may modulate IL6 gene methylation, particularly in males, and underscore the role of epigenetic mechanisms in the molecular pathways underlying gastric inflammation and disease progression, especially in high- prevalence populations. Editora / Evento / Instituição: Universidade Federal da Bahia Tipo: Dissertação 2025-07-11T00:00:00Z https://repositorio.ufba.br/handle/ri/42434 Título: Avaliação das citocinas IL-2, IL-12, IFN-y e TNF-a como preditores de mortalidade em pacientes com COVID-19 en estadi grave admitidos à unidade de terapia intensiva Autor(es): Brito, Gabriel Carvalho Primeiro Orientador: Melo, Fabrício Freira de Abstract: COVID-19, caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS CoV-2), has caused numerous clinical manifestations that can lead to death. In a short period of time, the disease spread globally, mainly affecting individuals with comorbidities such as hypertension and diabetes. Studies demonstrate the participation of the individual's immune system in the patient's clinical evolution as well as its outcome and, therefore, the study addresses the role of certain cytokines in regulating the immune response and the comparison between them. This is a cross-sectional study, approved by the National Research Ethics Commission (CONEP) and aimed to evaluate the serum levels of Th1 response cytokines (IL-2, IL-12, INF-y and TNF-a) hospitalized. in serious condition in an intensive care unit at the Hospital Geral of Vitória da Conquista in state of Bahia. The study was carried out on 56 patients with an average of 64 years old, ranging between 16 and 97 years old, with the majority of patients being female (58.3) and, as a result, it was applied that plasma concentrations of IL-2 and INF -y are present in patients affected by COVID-19 in serious condition. Editora / Evento / Instituição: Universidade Federal da Bahia, Instituto Multidisciplinar em Saúde Tipo: Dissertação 2024-08-09T00:00:00Z