DSpace Coleção:https://repositorio.ufba.br/handle/ri/205912024-03-24T12:38:51Z2024-03-24T12:38:51ZAvaliação da expressão gênica de osteoblastos cultivados em biomateriais (pla, pc-iso, abs m30i)Santos, Rebeca Pereira Bulhosahttps://repositorio.ufba.br/handle/ri/389862024-02-01T19:39:00Z2023-12-15T00:00:00ZTítulo: Avaliação da expressão gênica de osteoblastos cultivados em biomateriais (pla, pc-iso, abs m30i)
Autor(es): Santos, Rebeca Pereira Bulhosa
Primeiro Orientador: Trindade, Soraya Castro
Abstract: Some orofacial injuries are related to the loss of bone continuity and, consequently, to structural and functional damage that impair quality of life. The production of biological tissues from cell cultures is suggested as an auxiliary strategy for orofacial reconstructive surgeries, but the effectiveness of the technique depends on a support structure that guides the formation of functional tissues according to the receptor bed. In this context, the gene expression signature of osteoblastic cells (SAOS-2) cultured on 5 and 10 days in rigid matrices produced with a three-dimensional printer was investigated. To this end, osteoblasts cultured on polylactic acid polycarbonate (PLA), acrylonitrile-butadiene-styrene (ABS M30i) and Polycarbonate-ISO (PC-ISO) scaffolds were evaluated by Array Real time PCR system to determine the expression of genes related to osteoblastic differentiation, bone mineralization, ossification, growth factors, and transcription factors involved in inflammation and healing. The results obtained showed that the ABS-M30i scaffolds, with emphasis on the genes bmp5, bmp6, col9A2, col11A1, spp1, Ibsp, fgf2, vegfA, smad4, smad9, runx2 / bmpr1A, fgfr1 and Igfr1, the PC-ISO with emphasis on in the genes bmp5, bmp6, col19A1, col11A1, cfs2, Ibsp, fgf2, vegfa, smad1, runx2, bmPr1a and Fgfr and the PLA, highlighting the genes bmp5, bmp6, col19A1, col11A1, cfs2, spp1, minpp1, ibsp, fgf2 , tgfb2, smad1, smad5, smad4, bmpr1 and fgr1, allow the expression of a large number of genes responsible for bone formation in vitro, however, PLA seems to have allowed more exuberant expression in the genes that were highlighted, and PC-ISO allowed a more uniform overall response. Thus, the use of the three biomaterials in tissue engineering becomes promising.
Editora / Evento / Instituição: Universidade federal da bahia
Tipo: Tese2023-12-15T00:00:00ZAssociação de variantes genéticas nos genes DAD1 e OXA1L com asma e atopia em uma população adultaPires, Anaque de Oliveirahttps://repositorio.ufba.br/handle/ri/383722024-01-29T17:14:38Z2023-08-31T00:00:00ZTítulo: Associação de variantes genéticas nos genes DAD1 e OXA1L com asma e atopia em uma população adulta
Autor(es): Pires, Anaque de Oliveira
Primeiro Orientador: Figueirêdo, Camila Alexandrina Viana de
Abstract: Asthma is a highly complex immune-mediated disease, characterized by a reversible and intermittent obstruction of the airways that, despite having a higher prevalence in childhood, has shown a high incidence and mortality in adults. In recent years, several genomic wide association studies (GWAS) have identified a significant number of genetic variants responsible for asthma susceptibility. These variants have been shown to play an important role in the regulation of gene expression and in the heritability of asthma, including variants in DAD1 e OXA1L genes. The DAD1 gene is known for its role in the regulation of programmed cell death, and OXA1L is described for its involvement in mitochondrial biogenesis and oxidative phosphorylation. The present study aimed to identify new variants in the DAD1 and OXA1L genes and to evaluate the association with asthma and atopy markers in adults. The study involved the participation of 1,084 adult individuals divided into mild to moderate asthma, severe asthma and healthy controls belonging to a case-control study cohort of the Programa para Controle da Asma na Bahia-ProAR. Analyzes of associations between variants in the studied genes and asthma or atopy were performed using a multivariate logistic regression model adjusted for sex, age, body mass index (BMI), smoking, forced expiratory volume in 1 second (FEV1) and ancestry (PC1) using PLINK 1.9 software. Additive, dominant and recessive genetic models were used for all analyzed variables. In this study, new variants in the DAD1 and OXA1L genes that had never been described before were identified. The C allele of rs200470407 in OXA1L was negatively associated with lack of asthma control and increased IL-13. The A allele of rs61972400 and the C allele of rs76050305 in DAD1 were positively associated with airway obstruction, in addition to being in total linkage disequilibrium. In silico gene expression analyzes demonstrated that some of the polymorphisms in both genes are able to affect their respective levels of gene expression. Thus, our findings demonstrate that variants in the DAD1 and OXA1L genes may influence asthma and atopy in Brazilian adults.
Editora / Evento / Instituição: Universidade Federal da Bahia
Tipo: Tese2023-08-31T00:00:00ZAvaliação funcional de polimorfismos nos genes EBI3 e IL12A associados ao desenvolvimento de asma em uma coorte de crianças no município de Salvador-BaNascimento, Regina Santoshttps://repositorio.ufba.br/handle/ri/380462023-11-09T13:55:12Z2023-08-08T00:00:00ZTítulo: Avaliação funcional de polimorfismos nos genes EBI3 e IL12A associados ao desenvolvimento de asma em uma coorte de crianças no município de Salvador-Ba
Autor(es): Nascimento, Regina Santos
Primeiro Orientador: Figueiredo, Camila Alexandrina Viana de
Abstract: Asthma is a chronic inflammatory disease of the airways, non-transmissible, influenced by environmental and genetic factors. Allergic asthma is the most common phenotype of the disease and is associated with an exacerbation of the Th2 immune response. Several cell types are involved in the regulation of the immune system in the lung, with emphasis on regulatory cells Treg and Breg. Several inhibitory controls included by these cells have already been admitted, among which are the release of suppressive cytokines such as IL-10, TGF-β and IL-35. IL-35 is a heterodimeric cytokine, composed of the EBI3 and IL12p35 subunits, which are encoded by the EBI3 and IL12A genes, respectively. Changes in the levels of this cytokine have been associated with asthma and atopy. The consternation of the genetic component in the development of asthma is widely reported in the literature. In this context, given the importance of Treg cells and genetic susceptibility, this study set out to investigate the functional impact of polymorphisms in the regulatory genes EBI3 and IL12A in a population of Brazilian children. DNA from 1.218 children was genotyped using the Illumina 2.5 Human Chip Omni Bead. Logistic regression analyzes were performed using PLINK 1.9 software to verify the association between polymorphisms in EBI3 and IL12A, asthma and atopic markers, adjusted for sex, age, helminth survivors and ancestry markers. mRNA expression was performed using real-time qPCR. A total of 4 markers for IL12A and 5 for EBI3 were found. The surprising results that the C allele of rs2243131 in IL12A was positively associated with asthma (OR 1.35, CI 1.06–1.71), asthma severity (OR 1.36, CI 1.02–1.81), positive skin test for Blatella germanica (OR 1.59, CI 1.09–2.22), and also positively associated with the spontaneous production of IL-5 (OR: 1.71; CI: 1.11–2.62). The A allele of rs568408 in IL12A was also positively associated with a positive skin test for B. germanica (OR 1.65, CI 1.10-2, 37). rs582537 in IL12A was associated with a positive skin test for B. germanica (OR 0.64, CI 0.42-0.98) and Dermatophaoides pteronyssinus (OR 0.77, CI 0.60-0.98), in addition to being associated with the natural production of INF-y (OR : 0.52; CI: 0.52–0.99). With regard to EBI3, all the variants found were incorporated into atopy markers: the C allele of rs78749916 (OR 0.61, CI 0.40-0.93), the G allele of rs77145509 (OR 0.66, CI 0.46-0.94), and the A allele of rs76353132 (OR 0.68, CI 0.43-0.96), were associated with a positive skin test for Periplaneta americana. The rs76353132 variant was also associated with spontaneous INF-y production (OR: 0.72; CI: 0.52-0.99). The rs428253 C allele was associated with a positive skin test for at least one allergen (OR: 0.64; CI: 0.44–0.92), and the rs4905 G allele was associated with a positive IgE for at least one allergen (OR 0.62, CI 0 .40-0.95). IL12A mRNA expression levels were reduced in atopic asthmatic subjects when compared to controls. EBI3 mRNA expression levels were decreased in atopic asthmatic subjects compared to non-asthmatic and atopic subjects, and when compared to controls. In this study, we were able, for the first time, to describe new variants in the IL-35 regulatory pathway linked to asthma and atopy, highlighting the importance of immune regulation in the pathogenesis of asthma.
Editora / Evento / Instituição: UNIVERSIDADE FEDERAL DA BAHIA
Tipo: Tese2023-08-08T00:00:00ZEstudo de associação de variantes do gene BDNF com fenótipos de asma e atopia em uma população brasileiraCerqueira, Jessica Vieirahttps://repositorio.ufba.br/handle/ri/380272023-11-09T10:28:17Z2023-08-07T00:00:00ZTítulo: Estudo de associação de variantes do gene BDNF com fenótipos de asma e atopia em uma população brasileira
Autor(es): Cerqueira, Jessica Vieira
Primeiro Orientador: Figueirêdo, Camila Alexandrina Viana
Abstract: Asthma is one of the most common noncommunicable diseases in the world, affecting from 1 to 29% of the population in different countries. The inflammatory process of asthma is associated with immune system activation, airway hyperresponsiveness, epithelial cell activation, mucus overproduction, and airway remodeling. It is well recognized that Th2 cells are the main drivers of eosinophilic allergic airway inflammation, generating abundant amounts of IL-4, IL-5, IL-9 and IL-13. However, asthma in adults is more often non-allergic than allergic. Although inflammation appears as a key feature of the exacerbated response in asthma, anti-inflammatory therapy only reduces this exaggerated response but is not able to suppress it, leading to the hypothesis that other factors besides the inflammatory process contribute to the symptoms. observed in asthma. Barrier tissues, such as the lungs, are innervated by the peripheral nervous system, which detects stimuli, including harmful ones, and responds to them by regulating autonomic reactions. The nervous system in the tract plays an important role in regulating mucus intensity, vascular permeability, airway smooth muscle tone, and blood flow. Brain-Derived Neurotrophic Factor (BDNF) is a neurotrophin present in the lower airways and may contribute to changes in the structure and function of the airways. Individuals with asthma were selected from the patient cohort of the Program for Asthma Control in Bahia (ProAR). Peripheral blood samples were collected for cell counting, laboratory tests and DNA removal. Serum was stored for subsequent IgE and specific cytokine assays. All subjects underwent skin prick testing (SPT) for common allergies. The objective of this research was to evaluate the impact of variants in the BDNF gene on asthma and atopy phenotypes. The results showed that the G allele of rs962369 was associated with asthma levels (OR 0.74, 95% CI 0.564-0.985, p= 0.038). The A, G, G, G, T and A alleles of rs6265, rs11030104, rs7103411, rs988748, rs10767664, rs2030323, respectively, were specific associated with lack of reversibility. The G allele of SNVs rs7124442 was significantly associated with lower odds of atopy (OR 0.75, 95% CI 0.575-0.989, p= 0.041). While the G allele of SNV rs2030324 and the A allele of rs7934165 were associated with a greater chance of atopy (OR 1.26, 95% CI 1.048-1.516, p= 0.014) and (OR 1.25, 95% CI 1.041 - 1.506, p = 0.016). The G allele of rs7124442 and the A allele of rs11030119 were associated with a greater chance of exacerbation (OR = 1.575, Pperm = 0.037 and OR = 1.823, Pperm = 0.029). Furthermore, the G allele of the rs7124442 SNVs was associated with reduced IL-5 and IgE levels and associated with increased neutrophils in the blood. These data show that variants of the BDNF gene have an impact on asthma and also on atopy in our population.
Editora / Evento / Instituição: Universidade Federal da Bahia
Tipo: Tese2023-08-07T00:00:00Z